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. 2015 May 8;15:374. doi: 10.1186/s12885-015-1363-1

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© Pignochino et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Synergistic anti-proliferative effects of sorafenib and everolimus on MES-MM98 cell lines, calculated according to the number of viable cells in culture based on quantitation of the ATP present. (A) Representative dose-effect curve of MES-MM98 cells treated with scalar doses of sorafenib, everolimus or their combination. The dose effect curve was calculated by CalcuSyn software after 72 h of treatment. (B) Photograph of a representative colony growth of MES-MM98 cells after 10 day-incubation in complete medium alone (NT) or with sorafenib (SOR 2.5 μM), everolimus (EV 10 nM), or their combination (SOR 2.5 μM + EV 10 nM). (C) Analysis of the total surface area occupied by colonies/well after treatment with escalating doses of sorafenib (0, corresponding to control, 1.25 μM and 2.5 μM), alone or in combination with everolimus (10 nM). *p < 0.05 vs NT; †, p < 0.05 vs both single agents and control. Results are expressed as mean ± standard deviation of triplicate experiments.