Fig. 1.

Tuberin was phosphorylated at threonine 1462 via the Akt–PTEN pathway in the frontal cortex of AD and PD/DLB patients. Total cell lysates were subjected to SDS-PAGE and then immunoblotted with antibodies to p-tuberin, tuberin, p-Akt, Akt, p-PTEN, and PTEN. The blots were also probed with antibodies to PINK1 a neuronal specific marker for cortex. Separate blots with 50 μg protein were run for each set such as for p-tuberin and tuberin, p-Akt and Akt, and p-PTEN and PTEN. The blots are representative of at least three different experiments. The panel on the right shows a quantitative analysis of the blots shown in the figures. Mean ± SEM and *,** denotes P < 0.05 as compared to controls and as compared to PD respectively