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. Author manuscript; available in PMC: 2015 May 13.
Published in final edited form as: Neurochem Res. 2008 Mar 5;33(6):1113–1116. doi: 10.1007/s11064-007-9558-8

Fig. 2.

Fig. 2

Tuberin was phosphorylated at threonine 1462 via the Akt–PTEN pathway in the cortex of MPTP induced acute mouse model of PD. Total cell lysates were made from frontal cortex of mice (pooled from n = 4) treated with MPTP and sacrificed at various time points (0, 4, 12, 24, 48, 72, 96 h and 1 week). These lysates were subjected to SDS-PAGE and then immunoblotted with antibodies to p-tuberin, tuberin, p-Akt, Akt, p-PTEN and PTEN. The blots were also probed with antibodies to PINK1 a neuronal specific marker for cortex. Separate blots with 50 μg protein were run for each set such as for p-tuberin and tuberin, p-AKT and AKT, and p-PTEN and PTEN. The blots are representative of at least three different experiments