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. Author manuscript; available in PMC: 2016 Jan 8.
Published in final edited form as: Mol Cell Neurosci. 2015 Jan 8;64:116–122. doi: 10.1016/j.mcn.2014.12.007

Figure 3. Glutamate treatment results in Opa1 and cytochrome c release from the mitochondria to the cytosol.

Figure 3

HT22 cells were exposed to 5mM glutamate for 4 and 10 hours. Cells were fractionated to isolate a cytosolic and a mitochondrial/heavy membrane fraction and equal amounts of protein were resolved by SDS-PAGE. Membranes were probed with antibody against Opa-1 and cytochrome c (A). At 10 hours of glutamate treatment, there was a significant increase in Opa-1 (p<0.05; B) and cytochrome c (p<0.05; B) compared to controls. Immunofluorescence of HT22 cells (C) control and treated with 5mM glutamate for 10 hours, ATP synthase labeled red and cytochrome c labeled green, DAPI labeled blue. We measured Drp1 levels in both cytosolic and mitochondrial/heavy membrane fractions of control and 5mM glutamate exposed cells for 10 hours. There was no significant change in Drp1 at 10 hours glutamate exposure (p<0.05; D).