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. 2015 May 13;5:10132. doi: 10.1038/srep10132

Figure 7.

Figure 7

RARB and Nur77 co-operation regulate HSD3B2 in H295R cells. H295R cells were transfected with the human −1.05 kb HSD3B2 promoter constructs along with an expression vector RARB, Nur77 and in combination of RARB/Nur77. Transfection medium was changed after 6 h and cells were cultivated in either serum free or in normal growth medium for 24 h. Thereafter, cells were grown in the presence or absence of ATRA for another 24 h. (A) RARB and Nur77 co-operation study under serum starvation condition. (B) RARB and Nur77 co-operation study under normal growth condition. Promoter activation was assessed by the dual luciferase assay (Promega) using pRL-TK as internal control. Data are expressed in Relative Light Units (RLU). Error bars show the mean ± SD of four independent experiments. * p < 0.05, ** p < 0.01.