Figure 3.

Identification of the stem proteins by the in-solution and in-gel methods. (A) Multivariate correlation analysis of the number of peptide hits for the 75 stem proteins that identified by the in-solution method from analyzing the three replicate samples. (B) Venn diagram for the number of stem proteins, and (C) Venn diagram for the number of cell wall proteins identified by in-solution and in-gel methods coupled with LC-MS/MS analyses, respectively. A comparison of (B) vs. (C) indicates that the number of cell wall proteins accounts for around 10% of total stem proteins identified both in-solution and in-gel methods, which is a reflection of the fact that soluble proteins are extracted from the whole Arabidopsis stems.