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. 2015 May 13;5:10217. doi: 10.1038/srep10217

Figure 1. Purification of hADSCs from adipose tissue using the hybrid-membrane migration method.

Figure 1

(a) Procedure of the hybrid-membrane migration method. The primary adipose tissue cell solution (SVF) was filtered through the membranes. Subsequently, a washing solution identical to the culture medium was passed through the membranes. The membranes were removed from the membrane holder and inserted into dishes containing culture medium. hADSCs migrated out from the membranes and were expanded in the dishes during culture. (b) Morphology of PLGA/silk-10% (i), polyurethane (PU-11) (ii), nylon mesh filter (NY-11) (iii) and nitrocellulose (NC-8) (iv) membranes used for the hybrid-membrane migration method. The scale bars indicate 100 μm (i) and 40 μm (ii-iv). (c) The morphology of the primary adipose tissue cells (SVF) (i), SVF cells cultured on untreated PS dishes (ii) and on TCPS dishes (iii) for 8 days, and the cells that migrated from PLGA/silk-10% membranes and were subsequently cultured for 15 days after SVF was permeated through the membranes (iv). The scale bars indicate 100 μm.