Skip to main content
. 2015 May 13;5:10217. doi: 10.1038/srep10217

Figure 4. Pluripotency of hADSCs isolated using the hybrid-membrane migration method.

Figure 4

(a) Relative gene expression levels of Oct4 (i), Sox2 (ii), Nanog (iii) and Klf4 (iv) as analyzed by qRT-PCR in the primary adipose tissue cells (SVF), the primary adipose tissue cells (SVF) cultured on untreated PS and TCPS dishes for 8 days (passage one), the cells that migrated out from PLGA/silk-10%, PU-11, NC-8 and NY-11 membranes and were subsequently cultured on untreated PS (P) and TCPS (T) dishes after SVF was permeated through the membranes, hESCs (WA09) and hiPSCs (HS0077). The gene expression was standardized to the expression in the primary adipose tissue cells (SVF) cultured on TCPS. (b) The expression of the pluripotency protein Oct4 in the primary adipose tissue cells (SVF) cultured on untreated PS dishes (i), the primary adipose tissue cells (SVF) cultured on TCPS dishes for 8 days (passage one) (ii), and the cells that migrated out from NY-11 (iii) and PLGA/silk-10% (iv) membranes and were subsequently cultured on untreated PS dishes after SVF was permeated through the membranes, as analyzed by immunostaining for Oct4. Oct4 expression is shown in green, and nuclear staining by Hoechst is shown in blue. The scale bars indicate 100 μm.