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. 2015 May 13;6:74. doi: 10.3389/fendo.2015.00074

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Copyright © 2015 Garofalo, Capristo, Mancarella, Reunevi, Picci and Scotlandi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Analysis of NT157 effects on cell cycle after 48 h of treatment (1–3 μM) in MG-63 and U-2OS OS cell lines. Columns show the mean percentage of cells in different cell cycle phases as measured by flow cytometry. (B) Analysis of major downstream signaling of IRS-1 after treatment with or without NT157 (1–3 μM) by western blotting using 40 μg of total protein cell lysate. GAPDH was used as a loading control. The figure shows data representative of two independent experiments.