Figure 5.
Coordinated action of U2AF65 and U2AF35 in regulated splicing. (a) Western blotting analysis of RNAi-mediated U2AF65 and U2AF35 knockdown, showing reduced U2AF35 in U2AF65 siRNA–treated cells. β-actin is a loading control. (b) Splicing response of representative genes in response to RNAi against U2AF65 or U2AF35. Error bars, s.d. (n = 3 cell cultures). Uncropped gels are provided in Supplementary Data Set 1. (c) Global concordance of U2AF65- and U2AF35-dependent splicing revealed by RASL-seq. P < 2.2 × 10−16 by F test (n = 208 splicing-changed events). ΔPSI, difference in percentage spliced in, in HeLa cells treated with U2AF65 siRNA, U2AF35 siRNA or negative control (NC) siRNA.