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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Nat Neurosci. 2014 Nov 24;18(1):17–24. doi: 10.1038/nn.3886

Figure 1.

Figure 1

Combinations of transcription factors result in nociceptor production. (a) Few tdTomato, Tuj1-positive neurons are produced by the combination of six factors (6 TFs): Brn2, Ascl1, Myt1l, Ngn1, Isl2 and Klf7. (b) Removal of Brn2 markedly increases the number of tdTomato, Tuj1-positive neurons. (c–g) Omission of Ascl1 (c), Myt1l (d), Ngn1 (e), Isl2 (f) or Klf7 (g) from the six factors disrupts the generation of nociceptor neurons. Representative images for each transcription factor drop out were taken from n=4 wells from two separate transductions. Scale bars: 100 µm. (h) Quantification of the single factor dropout studies from n=4 wells from each of two separate independent transductions for each category.