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. Author manuscript; available in PMC: 2015 Jul 1.
Published in final edited form as: Nat Neurosci. 2014 Nov 24;18(1):17–24. doi: 10.1038/nn.3886

Figure 5.

Figure 5

Sensitization of induced nociceptors treated with the inflammatory mediator PGE2 and the chemotherapeutic drug oxaliplatin. (a) Sample calcium imaging recordings of induced nociceptors treated with 300 nM capsaicin before and after treatment with 1 µM PGE2 from recordings of n=41 tdTomato-positive / KCl-responding cells. (b) Plot of individual and mean response amplitudes for initial and PGE2-sensitized capsaicin treatments (paired t-test p=1.5×10−4). (c) Plot of initial versus PGE2-sensitized capsaicin response amplitudes for individual induced neurons. (d) Sample traces from extracellular multi-electrode array recordings of induced neurons in response to 300 nM capsaicin following 10 minute exposure to vehicle control (n=5 MEAs) or oxaliplatin (50 µM; n=4 MEAs) on induced neurons from two separate transductions. (e) Quantification of spikes per minute from induced nociceptors in response to capsaicin alone (control) and capsaicin following oxaliplatin treatment. Error bars are ± SEM.