Fig. 5.
Immunofluorescent images of VDR localization (nuclear vs plasma membrane). Representative image of VDR and DAPI detection in skeletal muscle cross-sections of a single subject at baseline (a) and after 16 weeks of vitamin D3 supplementation (b). A software algorithm was utilized to colocalize the total VDR signal area (red) and myonuclei (blue DAPI) across individual field images of skeletal muscle (Color figure online)
