Figure 1.

Generation of NK clones from individual NK cells with specific KIR receptor repertoires. (A) Flow cytometry representation of NK-cell subsets defined by a combination of four anti-KIR mAbs. In this example, subsets used for FACS-assisted single-cell deposition express 2DS1, either alone (subset 1, red) or in combination with at least one receptor among 2DL2, L3, and S2 (subset 2, green) or 3DL1 (subset 3, blue). The percent frequency of NK-cell subsets is indicated. (B) Representative NK clones obtained after 3-week in vitro propagation in the presence of IL-15 trans-presentation. E115: 2DS1^pos; E78: 2DS1^pos/CH-L^pos; E86: 2DS1^pos/3DL1^pos. For E78, specific KIR(s) can be identified by real-time RT-qPCR.