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. 2015 May 13;6:230. doi: 10.3389/fimmu.2015.00230

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Copyright © 2015 Pittari, Filippini, Gentilcore, Grivel and Rutella.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protocols to generate CIK cells from PBMCs. (A) Current protocols used to differentiate CIK cells from PBMCs rely on IFN-γ, anti-CD3 mAbs or thymoglobulin (TG), and IL-2. (B) A representative experiment depicts the phenotype of CIK cells after 3-week culture under the above cytokine conditions.