Table 1.

N-terminal glutamine to pyroglutamate conversion rates (%/h) for PrP peptides (see Figure 1 for sequences) in various media at 8°C.

		Q86-K109	Q95-K109	Q101-K109	Q189-K197	Q189-K207	Q189-R211
PTB-FA	Slope	0.14 ± 0.01	0.13 ± 0.01	0.11 ± 0.003	0.08 ± 0.002	0.09 ± 0.003	0.08 ± 0.01
	R2	0.8745	0.9692	0.9778	0.9673	0.9689	0.7083
PTB-HCl	Slope	0.18 ± 0.01	0.18 ± 0.01	0.17 ± 0.01	0.14 ± 0.01	0.15 ± 0.005	0.21 ± 0.02
	R2	0.9307	0.9428	0.8782	0.9529	0.9776	0.9109
PTB-water	Slope	−0.05 ± 0.06	0.009 ± 0.004	0.004 ± 0.001	0.002 ± 0.003	0.001 ± 0.002	0.002 ± 0.008
	R2	0.1004	0.2997	0.3031	0.02758	0.03721	0.005692
Water	Slope	No data	0.23 ± 0.02	0.01 ± 0.01	0.02 ± 0.002	0.03 ± 0.002	0.13 ± 0.02
	R2		0.8041	0.06837	0.7628	0.9538	0.5798

PTB, post-trypsin buffer.