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. 2015 Jun;150:54–63. doi: 10.1016/j.jsbmb.2015.03.010

Fig. 6.

Fig. 6

Regulation of STS mRNA by IL1α in SKOV3 cells. (A) Dose-dependent effect of IL1α. Cells were treated with increasing concentrations of IL1α for 48 h. Levels of mRNA were standardized to 18S rRNA and presented as fold increase relative to the positive control at each dose point (P < 0.01, F = 18.67, df = 3, 2, one-way ANOVA with Tukey’s post-hoc tests. * = P < 0.05; ** = P < 0.01). (B) Time-dependent effect of IL1α. Cells were treated with 0.5 ng/ml IL1α for times indicated. Levels of mRNA were standardized to 18S rRNA and presented as fold-increase relative to the corresponding untreated control at each time point (P < 0.01, F = 69.15, df = 1, 12, two-way ANOVA with Tukey’s post-hoc tests *** = P < 0.001). (C) Inhibition of IL1α-stimulated STS mRNA expression by IL1RA. Cells were cultured for 48 h in absence and presence of IL1α (0.5 ng/ml), without or with IL1RA (25 ng/ml). Total RNA was standardized to 18S rRNA and presented as fold increase relative to untreated control (P < 0.001, F = 36.64, df = 3, 2, one-way ANOVA with Tukey’s post-hoc tests **P < 0.01; ***P < 0.001). Bars and data points indicate mean ± SEM of 3 independent experiments.