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COH-29 treatment activates DNA damage checkpoint. (A) The effect of COH29 treatment on DNA damage checkpoint proteins in HCC1937 and HCC1937 + BRCA1 human breast cancer cells assessed by Western blot. (B) The effect of HU on DNA-damage response (DDR)–associated proteins was assessed by Western blot analysis. Cells were treated with COH29 at the indicated doses for 24 hours, and cell lysates were subjected to immunoblotting using the indicated antibodies.
