Fig. 6.
Effects of attenuation of plasma membrane PtdIns(4,5)P2 hydrolysis on BRET curves between AT1-R and Rab5 or β-arrestin2. HEK293 cells were transfected with plasmids encoding DN-GRK2, AT1-R–Rluc, and Rab5-YFP. After 24 hours, the cells were exposed to either 100 nM AngII (red trace), 1 μM TRV120023 (TRV3; blue trace), or vehicle (dashed lines) at the indicated time point. The BRET records are the average of three independent experiments. Mean values ± S.E.M. are shown (n = 3). Stim, stimulation.