Figure 1.

Vector biodistribution in the injected forelimb and at distance. (a) Vector copy number per diploid genome in each muscle of the injected forelimb of each rAAV-injected dog at euthanasia. A total of 13 muscles were analyzed in the injected limb. Each point represents the vector copy number per diploid genome in each muscle of the injected limb. Empty symbols were used for the groups of dogs injected with 40% of the limb volume (groups 1, 2, and 3), and full symbols were used for the groups of dogs injected with 20% of the limb volume (groups 4, 5, and 6). The small horizontal bars represent the mean of the values obtained for each dog. No signal was detected (<0.004 vg/dg) in the muscles of the control dogs. CV, coefficient of variation within the injected forelimbs. (b) Vector copy number per diploid genome in different tissues of the different groups of dogs at euthanasia. For each dog, the analysis was performed on 13 muscles of the injected forelimb, on 13 muscles of the noninjected forelimb, on 20 muscles at distance (muscles of hind limbs (n = 2), muscles of the body (n = 15), heart (n = 2) and diaphragm), on spleen (n = 1), on testis (n = 2), on kidney (n = 1), on liver (n = 3), on draining lymph nodes (n = 2), and on other lymph nodes at distance (n = 5). Each bar of the histogram represents the mean (with standard deviations) of the results obtained in the different dogs of a same group. Striped bars were used for the groups of dogs injected with 40% of the limb volume (groups 1, 2, and 3). Full bars were used for the groups of dogs injected with 20% of the limb volume and a flow rate of 10 ml/minute (groups 4 and 5), and punctuated bars were used for the groups of dogs injected with 20% of the limb volume and a flow rate of 35 ml/minute (group 6). No signal was detected (<0.004 vg/dg) in the tissues of the control dogs (data not shown).