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. Author manuscript; available in PMC: 2015 May 13.
Published in final edited form as: Toxicol Appl Pharmacol. 2012 Apr 19;262(1):11–21. doi: 10.1016/j.taap.2012.04.014

Figure 3.

Figure 3

Carcinogenic metals As(III) and Cr(VI) in drinking water changed the colorectal proteome expression profile. (A) 2D-DIGE of colorectal proteins. Each individual sample (As, Cr, and control) and a pooled reference sample were labeled with Cy5 and Cy3, respectively, mixed, and separated on a 2D-PAGE gel. Gels were scanned and a set of Cy5 and Cy3 images were obtained from each gel. An overlay of two dye scan-images was obtained. (B) Spots with a student’s t-test p value less than 0.05 in As(III)- or Cr(VI)- treated group, are shown in circles and number according to pI and Mw. Thirty six protein spots with significant change in their levels (p < 0.05; fold change ≥ ±1.5) were cut out of this gel and subjected to tryptic digestion followed MS analysis.