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. 2015 Jan 27;5:3. doi: 10.1186/2045-3701-5-3

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© Wang et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Characterization of ADSCs and the effect of AGE-HSA on CD105 and CD90 expression in ADSCs. (A) Representative image of isolated ADSCs on the first day. (B) Adherent ADSCs 3 days after plating, showing a spindle shape. (C) After 10 days, most of the ADSCs exhibited fibroblast-like morphologies and had reached near-confluence. Scale bar is equivalent to 50 μm. (D) Passage 3 ADSCs were labeled with antibodies or matching isotype controls against the indicated antigens and analyzed by flow cytometry to obtain representative immunophenotypes. FACS showed that ADSCs expressed CD105 and CD90, but not CD34, CD14, CD45 or HLA-DR. AGE-HSA altered CD105 and CD90 expression in ADSCs.