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. 2015 Jan 27;5:3. doi: 10.1186/2045-3701-5-3

Table 2.

Sequences of primers and PCR conditions

Primer sequence Product size (bp) Ta (°C)/cycles
RAGE S: 5′-CTGGTGTTCCCAATAAGG-3′ 372 58.5/38
AS: 5′-AGGTCAGGGTTACGGTT-3′
GAPDH S: 5′-ACCACAGTCCATGCCATCAC-3′ 452 56.0/26
AS: 5′-TCCACCACCCTGTTGCTGTA-3′

S, sense; AS, antisense; Ta, annealing temperature.