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. Author manuscript; available in PMC: 2015 May 13.
Published in final edited form as: Methods Enzymol. 2015 Jan 5;550:215–249. doi: 10.1016/bs.mie.2014.10.049

Figure 6.

Figure 6

Sequence-specific activation of Spinach.ST molecular beacons. Spinach.ST.2m (A) (for structure, see Fig. 3) and Spinach.ST (B) (for structure, see Fig. 1) flanked by tRNA and embedded within larger transcripts (for structure, see Fig. 4) were incubated with cognate or scrambled trigger oligonucleotides that were similarly flanked by tRNA and expressed as a portions of larger transcripts. Spinach molecular beacons were activated only by cognate trigger-containing transcripts and led to the accumulation of DFHBI fluorescence over time.