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. Author manuscript; available in PMC: 2015 May 13.
Published in final edited form as: Methods Enzymol. 2015 Jan 5;550:215–249. doi: 10.1016/bs.mie.2014.10.049

Figure 7.

Figure 7

Schematic of trigger-generating primers for real-time signal transduction with NASBA. Regions on the amplicon that are involved in primer binding (PBS-F and PBS-R), associative toehold formation (domain D), and partial Spinach.ST trigger generation (domains 2 and 5) are depicted as dark boxes. Hybridization is depicted using vertical slashes, while the potential hybridization of the PBS-F domain of the NF primer to the complementary strand is indicated with colons. Dashed regions in the target, double-stranded DNA transcription template and RNA transcript denote target sequences that are not involved in priming or in Spinach.ST activation.