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. Author manuscript; available in PMC: 2015 May 13.
Published in final edited form as: Methods Enzymol. 2015 Jan 5;550:215–249. doi: 10.1016/bs.mie.2014.10.049

Figure 9.

Figure 9

Potential real-time nucleic acid sequence-based amplification (NASBA) amplicon detection using universal Spinach.ST molecular beacons. A scheme for generating NASBA primers that upon transcription creates an intramolecular RNA fold that can activate a universal Spinach.ST trigger is depicted. Transcribed, primer-derived sequences are shown as black lines. Transcribed, target-derived sequences are shown as dashed lines. Regions D1 and D2 that are involved in intramolecular RNA stem–loop formation are highlighted as thick gray blocks. Hybridization is depicted using vertical slashes. The dark bars at the junctions of domains 2* and D1 and domains D2 and 5* designate possible complementary base pairs that might stabilize the associative trigger.