The impact of colchicine on the morphology of control neurons. (A–H) Representative morphology of C001 (A, B) or C002 (C, D) TH+ neurons treated with vehicle (A, C) or colchicine (1 nM for 14 days) (B, D) and quantification of their total neurite length (E), number of terminals (F), number of branch points (G) and Sholl analysis (H). (I–P) Representative morphology of C001 (I, J) or C002 (K, L) TH− neurons treated with vehicle (I, K) or colchicine (1 nM for 14 days) (J, L) and quantification of their total neurite length (M), number of terminals (N), number of branch points (O) and Sholl analysis (P). *, p < 0.001, vs. vehicle-treated C001 or C002, n = 52 TH+ neurons and n = 54 TH− neurons for each condition of C001 or C002 neurons, all from three independent experiments.