Figure 4.

AGR2 silencing decreases pancreatic cancer growth and metastasis in vivo. A, tumor growth of MPanc-96 cells stably expressing shControl or shAGR2-1 and also expressing the luciferase gene with and without gemcitabine treatment was assessed in nude mice. Bioluminescent imaging was used to estimate tumor volume. AGR2 silencing with and without gemcitabine significantly reduced the tumor volume. B, in vivo apoptosis was measured by TUNEL assay on paraffin sections from shControl and shAGR2-1 animals treated with gemcitabine. AGR2 silencing along with gemcitabine treatment significantly increased the apoptosis in tumor tissues. Columns, mean for five animals; bars, SE (*, P < 0.05 versus control).