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. Author manuscript; available in PMC: 2015 May 13.
Published in final edited form as: J Neurosci Res. 2009 Nov 15;87(15):3511–3519. doi: 10.1002/jnr.21981

Fig. 5.

Fig. 5

Treg depletion results in increased numbers of infiltrating CD4+ cells and increased numbers of IFNγ producing cells in the CNS. Mice treated with either rat IgG (n=3) or PC61 (n=3) were immunized with mouse MOG and sacrificed on day 20. (A) Isolated SC were sectioned and stained for the presence of CD4+ T cells. (B) Mononuclear cells from the CNS were gated on CD4+ cells and stained for the expression of CD62L or CD69. (C) Cells isolated as in (B) were stained with anti-CD4, anti-CD25, and anti-Foxp3. (D) ELISPOT analysis. CNS tissues from each group were pooled together and 105 cells were plated and stimulated with either mouse MOG or MOG35-55. Dashed bars- control IgG treated; open bars- PC61-treated animals. Data are expressed as stimulation indices and represent one of 2 independent experiments with three mice per group.