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. Author manuscript; available in PMC: 2015 May 13.
Published in final edited form as: J Neurosci Res. 2009 Nov 15;87(15):3511–3519. doi: 10.1002/jnr.21981

Fig. 6.

Fig. 6

Depletion of Tregs does not result in the emergence of T cells reactive to new encephalitogenic epitopes but does lead to increased proliferation to MOG35-55 in the periphery as well as an enrichment of CD4+TCRVβ8+Foxp3− T cells in the CNS. (A) Spleens or (B) LNs from individual mice treated with either rat IgG (hatched bars, n=3) or PC61 (open bars, n=3) and immunized with mouse MOG were isolated and pooled on day 10 post immunization. Cells were stimulated with different peptides of MOG extracellular domain. Data are expressed as stimulation indices and represent one of two independent experiments with three mice per group. * p<0.0002. (C) Mice treated with either rat IgG or PC61 and immunized with mouse or rat MOG were sacrificed on day 20 post immunization and cells from the CNS were collected. Cells were stained with anti-CD4, anti-TCRVβ8 and anti-Foxp3 antibodies.