Table I.
Increased Incidence and Disease Severity in Treg-Depleted Mice Immunized with Mouse, but not Rat, MOG
| Immunogen | Treatmenta | Incidence | Mean Day of Onset | Mean Maximum Disease Score | p Value | Day 40 Disease indexb |
|---|---|---|---|---|---|---|
| Mouse rMOG | Rat IgG | 11/15 | 14 | 1.8 | 0.035 | 172 |
| PC 61 | 14/15 | 14 | 2.8 | 397 | ||
| Rat rMOG | Rat IgG | 7/7 | 16 | 3.4 | 424 | |
| PC61 | 9/9 | 14 | 3.4 | 476 | ||
| Rat rMOG | Rat IgG | 3/5 | 19 | 1.4 | 135 | |
| No Boost | PC61 | 3/5 | 22 | 1.6 | 133 |
a
C57BL/6 mice that had received three i.p. injections of either rat IgG or PC61 were immunized by standard protocol with mouse MOG, rat MOG, or with rat MOG omitting boost, and observed daily for signs of clinical disease. Onset and scores were calculated for mice that developed clinical signs while maximum disease scores were calculated for all mice.
b
Disease indices for each group were calculated as: ((sum of the mean clinical scores)/(day of disease onset)) × 100.