(A) Construction of exogenous siRNA-resistant lnc-BATE1 mutant (lnc-BATE1_Exo) from the endogenous transcript (lnc-BATE1_Endo).
(B) Schematic illustration of procedure used for rescue experiments.
(C) Design of qPCR primer pairs and agarose gel image of the resulting PCR products. Lane 2: lnc-BATE1_Endo or _Exo amplified by P1 primer pair; lane 3: lnc-BATE1_Endo amplified by P2 primer pair; lane 4: lnc-BATE1_Exo amplified by P2M primer pair.
(D) Expression (top) and localization (bottom) of total lnc-BATE1 in brown adipocytes infected with GFP control viruses or with lnc-BATE1_Exo viruses prior to transfection with control DsiRNA (DsiC).
(E-G) Expression of endogenous or exogenous lnc-BATE1 (E), brown adipocyte markers (F) and general adipogenic markers (G) in brown adipocytes infected with GFP control virus or with lnc-BATE1_Exo virus prior to transfection with control DsiRNA (DsiC) or lnc-BATE1 DsiRNA (Dsi2).
Error bars are SEM., n =3. *P ≤0.05, **P ≤0.01.