Figure 3. Srsf2P95H mutation impairs hematopoietic stem cell self-renewal in a manner distinct from Srsf2 loss.
(A) Depiction of competitive bone marrow (BM) transplantation assay. (B) Percentage of CD45.2+ chimerism in the peripheral blood of recipient mice (n=10 mice/genotype). (C) Chimerism and (D) flow cytometric enumeration of CD45.2+ LSK (left) and myeloid progenitor (MP; lineage-negative Sca1-c-Kit+) (right) cells in BM of Mx1-cre Srsf2WT, Mx1-cre Srsf2fl/WT, Mx1-cre Srsf2fl/fl and Mx1-cre Srsf2 P95H/WT mice 14 weeks after pIpC injection. Error bars represent mean ± SD; **p< 0.001, ***p < 0.0002; ****p< 0.0001. See also Figure S3.