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. 2015 May 6;11:103. doi: 10.1186/s12917-015-0417-6

Figure 1.

Figure 1

ARV S1133 induces autophagy and subsequent apoptosis in cultured cells. (A) DF1 cells infected with ARV S1133 at an MOI of 20. (B) Vero cells infected with ARV S1133 at an MOI of 5 for 0–42 hr. At the indicated time points, cells were stained with monodansylcadaverine (MDC) or Hoechst 332588. The percentage of positive cells was calculated for 20 independent fields at a magnification of 200×. (C) Vero cells infected with ARV S1133 at an MOI of 5 for 0–36 hr. In Hoechst 33258-stained cells (bright blue), arrows indicate apoptotic nuclei with condensed chromatin. In MDC stained cells, arrows indicate the autophagic vacuoles (×400 magnification, scale bar 10 μm).