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. 2015 May 6;11:103. doi: 10.1186/s12917-015-0417-6

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© Lin et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Functional interaction between RhoA, ROCK and Beclin-1 in ARV S1133-infected cells (A, B). Vero cells pretreated with DMSO (left panel) or Y-27632 (right panel) for 4 hr then infected with ARV S1133 at an MOI of 5 for various incubation periods. 500 μg soluble cell lysates were precleared and immunoprecipitated with IP antibodies as indicated. The immunocomplexes were purified by protein A beads and loaded onto an SDS-PAGE gel. The interacting proteins were detected using the Western blotting technique.