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. 2015 May 8;6:7062. doi: 10.1038/ncomms8062

Figure 3. Acute stimulation of vHIP versus mPFC or AMY terminals in NAc promotes opposite behavioral responses to CSDS.

Figure 3

(a). Schematic of experimental time course. Representative traces validating that 4 Hz stimulation of vHIP (b) (scale bar 200 pA, 1 s), mPFC (c) (scale bar 50 pA, 1 s) and AMY (d) (scale bar 50 pA, 1 s) terminals in NAc slices evokes EPSCs. Blue squares indicate light pulse delivery. In vivo, 4 Hz stimulation of vHIP terminals in NAc shell (e) reduced time spent interacting with a target mouse (F1,39=4.274 interaction effect, P<0.05, post hoc *P<0.05, n=20,21) and (h) increased time spent in corners (F1,39=6.560 target effect, F1,39=4.271, stimulation effect, P<0.05, post hoc *P<0.05, n=20,21). Stimulation of mPFC terminals in NAc (f) increased time spent interacting with a target mouse (F1,26=9.174 stimulation effect, P<0.01, post hoc **P<0.01, n=13,15) and (i) decreased time spent in corners (F1,26=7.735, P<0.01, post hoc *P<0.05, n=13,15). Stimulation of AMY axons in NAc (g) increased time spent interacting with a target mouse (F1,14=5.4284 interaction effect, P<0.01, post hoc **P<0.01, n=8) but (j) did not affect time spent in corners. Two-way repeated-measures ANOVAs with Bonferroni post hoc tests. ‘No stim' and ‘4 Hz' denote non-stimulated controls versus 4 Hz stimulated mice. ‘No target' and ‘Target' indicate absence or presence of a target mouse during testing. Error bars represent s.e.m.