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. 2015 May 13;10(5):e0126577. doi: 10.1371/journal.pone.0126577

Fig 6. Inhibition effect of κ-carrageenan on SW731 mRNA and protein expression.

Fig 6

(A) MDCK cells were infected with SW731 (MOI = 3) for 1h, and then treated with 250 μg/ml of κ-carrageenan or PBS after virus internalization. After 6 h, total RNA was extracted and analyzed using qRT-PCR. (B) MDCK cells were infected with SW731 (MOI = 3), and then treated with 250 μg/ml of κ-carrageenan or PBS after virus internalization. After 8 h, Western blot was performed using anti-NP protein antibody and HRP-labeled second antibody. (C) MDCK cells were infected with SW731 (MOI = 3), and then treated with 250 μg/ml of κ-carrageenan or PBS after viral internalization. After 8 h, immunofluorescence staining was performed using anti-NP protein antibody and FITC-labeled second antibody, and NP positive cell percentage was measured. Results were analyzed with the independent sample t test (n = 3). Values are means ± SEM. Significance: *P < 0.05 vs. nondrug treated control group; **P < 0.005 vs. nondrug treated control group. Results are representative of two independent experiments.