Table 2. Enzyme activity of NA a .
K m (μM) | V max | |
---|---|---|
SW731 | 198.7±22.3 | 2.49±0.32 |
SW731+carrageenan | 237.9±52.1 | 2.08±0.24 |
aA standardized virus dose of 105 PFU/ml was used for the NA kinetics assay.
The enzyme kinetics data (± standard deviation) were fit to the Michaelis-Menten equation by nonlinear regression to determine the Michaelis constant (K m) and maximum velocity (V max) of substrate conversion (in fluorescent units per second).