Fig 7. Rev Overexpression Reverses the Repression of HIV-1 Gag by Tra2β.

HEK 293T cells were transfected with HIV-1 proviral clone pNL4-3 GagzipGFP in the presence of either plasmid expressing GFP or GFP-Tra2β with (+) or without (-) a Rev-expression vector. Forty-eight hours post transfection, cells were harvested and extracts analyzed on SDS-PAGE gels. GFP signals for GagGFP, GFP, and GFP-Tra2β were imaged directly using a Bio-Rad MP imager. Total protein loading was verified using Stain-Free gels (Bio-Rad). At top is a representative assay and bottom, a summary of results from n>3 independent assays.