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. 2015 May 13;10(5):e0125315. doi: 10.1371/journal.pone.0125315

Fig 8. Tra2β Depletion Reduces HIV-1 Env Protein Accumulation.

Fig 8

Cells containing an integrated, doxycycline-inducible HIV-1 provirus (HeLa rtTA HIVΔmls) were transduced with lentiviruses expressing either control (shNT) or shRNA targeting Tra2β(shTra2β) or Tra2α (shTra2α) and a cassette conferring puromycin resistance. Transduced cells were selected using puromycin for 48–72 h, then HIV-1 provirus expression induced by addition of doxycycline. Cell extracts were subsequently analyzed by western blot to (A) confirm Tra2β depletion, (B) examine alterations in HIV-1 Env expression by western blot, or (C) for changes in HIV-1 Gag levels by p24 ELISA. Shown is a summary of results obtained of n>3 assays. Asterisks indicated values deemed significant from control (shNT) at p<0.05.