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. 2015 May 13;10(5):e0126501. doi: 10.1371/journal.pone.0126501

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© 2015 Karakas et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) AMELX and AMELY gene products from the preamplification step, (B) water-in-oil emulsion droplets that include PCR reaction mix with allele specific primers for AMELX and AMELY genes, a single DNA molecule from the preamplification step, and a single primer-coated magnetic bead, (C) oil droplets each harboring either the AMELX or AMELY gene fragment, (D) beads coated with either AMELX or AMELY-specific DNA following the BEAMing reaction, (E) beads hybridized either with the Cy5-AMELY or FITC-AMELX probe, (F) flow cytometry result of a test sample. Green beads represent AMELX, red beads AMELY, and blue beads represent both AMELX and AMELY-specific DNA sequences.