Table 2. Body weight, liver weight, and blood chemistry results of mice fed a high-fat diet.

High-fat diet
	Female			Male
	Control	UBXD8-LKO	P value	Control	UBXD8-LKO	P value
Parameter
Body weight (g)	39.63±1.79	35.18±1.43	0.063	43.51±1.13	39.04±1.51	0.025*
Liver weight (g)	1.44±0.12	1.34±0.09	0.518	1.82±0.15	1.60±0.15	0.326
Liver/Body ratio (%)	3.61±0.19	3.79±0.16	0.512	4.17±0.30	4.03±0.24	0.729
Serum parameter
Total protein (g/dL)	4.78±0.04	4.89±0.09	0.309	4.77±0.05	4.68±0.07	0.332
Albumin (g/dL)	3.25±0.04	3.28±0.05	0.621	3.04±0.04	2.95±0.05	0.24
Total bilirubin (mg/dL)	0.060±0.007	0.094±0.008	0.005*	0.050±0.002	0.057±0.003	0.147
Glucose (mg/dL)	230.53±11.66	213.53±12.12	0.321	211.40±11.47	208.87±10.42	0.871
Triglyceride (mg/dL)	59.67±5.14	39.67±4.93	0.009*	76.73±6.28	48.47±6.89	0.005*
Phospholipid (mg/dL)	162.07±8.69	183.60±9.34	0.103	203.87±6.33	187.00±8.45	0.122
Free fatty acid (μEQ/L)	724.20±41.04	651.27±43.04	0.23	639.87±32.13	545.67±24.26	0.027*
Total cholesterol (mg/dL)	88.93±5.58	94.07±5.81	0.529	110.13±3.78	97.73±5.45	0.072
Esterified cholesterol (mg/dL)	69.07±4.27	69.87±4.50	0.898	84.20±2.85	73.87±4.05	0.046*
Free cholesterol (mg/dL)	19.87±1.37	24.20±1.41	0.037*	25.93±1.02	23.87±1.46	0.257
Aspartate aminotransferase (IU/L)	99.67±7.16	173.53±16.36	<0.0001*	89.20±11.23	115.80±11.87	0.115
Alanine aminotransferase (IU/L)	44.40±8.86	85.87±9.27	0.003*	57.93±17.46	54.60±12.76	0.879
Lactate Dehydrogenase (IU/L)	570.73±84.71	486.87±40.20	0.379	478.93±49.36	513.93±54.61	0.638
Alkaline phosphatase (IU/L)	280.80±12.96	624.80±80.54	<0.0001*	226.87±19.98	344.07±55.82	0.058

Comparison of control and UBXD8-LKO mice (30 wk old) fed a high-fat diet for 26 wk. For each group, 15 females and 15 males were examined. P values were obtained by non-paired Student's t test. Selected data sets are presented as box plots in Fig 3. n = 15. All data are presented as mean ± SE. P values are the results of non-paired Student's t test

(*: P<0.05).