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. 2015 May 13;10(5):e0126186. doi: 10.1371/journal.pone.0126186

Fig 4. Function expression of OsPT4 in yeast.

Fig 4

The yeast cells were grown in solution that had been stained for acidification. The pH indicator, bromocresol purple, did not change from blue to yellow until the yeast cells had significant growth in culture. The medium contained 0 μM and 75 μM Pi, respectively. A: Color shifts in wild-type (WT), yeast strain pho84 (control), and pho84+OsPT4, which express OsPT4 in pho84 grown on synthetic defined (SD) Ura mediums. B: Growth conditions of wild-type, pho84, and pho84 transformed with pYES2-OsPT4 generated in a 24-hour liquid culture under 0μM and 75μM Pi conditions. Statistically significant differences are indicated: **p<0.01, Student’s one-way ANOVA analysis.