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. 2015 May 13;10(5):e0123223. doi: 10.1371/journal.pone.0123223

Fig 2. Oxidative stress does not induce P-body accumulation in M1-THPs or M2-THPs.

Fig 2

(A) M1- and M2-polarized THP-1 cells, either untreated (NT) or treated with 0.5 mM arsenite (Ars) for 30 minutes, were fixed and stained with a goat polyclonal antibody specific for EDC4 and a mouse monoclonal antibody specific for Dcp1a, followed by staining with Alexa 488-conjugated donkey anti-goat IgG and Alexa 568-conjugated donkey anti-mouse secondary antibodies. (B) Bar graph showing the average number of EDC4- or Dcp1a- positive foci per cell. The fluorescent dots were counted as described in Methods, and the error bars indicate SD. **p<0.01, ***p<0.001