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. 2015 May 13;10(5):e0125253. doi: 10.1371/journal.pone.0125253

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© 2015 Guan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (a) Primary USCs exhibited a spindle-shaped morphology. Osteogenic differentiation was confirmed using Alizarin Red (b) and von Kossa (c) staining, (d) Toluidine blue staining revealed chondrogenic differentiation, and (e) adipogenic differentiation was verified using Oil red O staining. (f) Flow cytometric analysis demonstrates that USCs expressed markers associated with mesenchymal stem cells and did not express HLA-DR. Black traces indicate isotype controls and gray traces show the level of cell-surface expression. Scale bar = 400 μm.