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. 1992 May;89(5):1564–1570. doi: 10.1172/JCI115750

Plasma cholesterol transport in anhepatic rats.

S H Quarfordt 1, B Landis 1, G Cucchiaro 1, Y Yamaguchi 1, B Oswald 1
PMCID: PMC443030  PMID: 1569195

Abstract

The plasma appearance of newly synthesized cholesterol in anhepatic laboratory diet-fed rats was 10% of the intact rat. In intact rats this cholesterol was mainly ester in lower density lipoproteins, but for anhepatic rats it was virtually only free in high density lipoprotein. Chylomicron cholesterol ester was removed much more slowly from anhepatic than control plasma and returned primarily as free in high density lipoproteins, with the control return 10 times the anhepatic return. Lower density lipoprotein cholesterol ester transfer to an extravascular pool in anhepatic rats was less than 10% of controls. The liver was responsible for 95% of the extravascular lower density lipoprotein ester pool and only 50% of the for high density lipoprotein ester. Despite decreased anhepatic lipoprotein catabolism, the mass of both plasma low and high density lipoproteins progressively decreased indicating an even greater decrease in influx. The anhepatic fractional catabolic rate of apo A1 was similar to controls, but that of apo E was considerably less. Despite the unchanged catabolism of apo A1 and the reduced catabolism of apo E, plasma apo A1 decreased less than apo E after hepatectomy. The anhepatic data confirm the pivotal role of the liver in maintaining plasma low and high density lipoprotein cholesterol concentrations. They suggest that, in addition to its anabolic and catabolic functions, the liver also acts as a reservoir buffering changes in plasma concentration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABEL L. L., LEVY B. B., BRODIE B. B., KENDALL F. E. A simplified method for the estimation of total cholesterol in serum and demonstration of its specificity. J Biol Chem. 1952 Mar;195(1):357–366. [PubMed] [Google Scholar]
  2. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  3. BURSTEIN M., SAMAILLE J. [On a rapid determination of the cholesterol bound to the serum alpha- and beta-lipoproteins]. Clin Chim Acta. 1960 Jul;5:609–609. doi: 10.1016/0009-8981(60)90075-9. [DOI] [PubMed] [Google Scholar]
  4. Bilheimer D. W., Eisenberg S., Levy R. I. The metabolism of very low density lipoprotein proteins. I. Preliminary in vitro and in vivo observations. Biochim Biophys Acta. 1972 Feb 21;260(2):212–221. doi: 10.1016/0005-2760(72)90034-3. [DOI] [PubMed] [Google Scholar]
  5. Bollman J. L., Van Hook E. A simplified two-stage hepatectomy in the rat. J Appl Physiol. 1968 May;24(5):722–723. doi: 10.1152/jappl.1968.24.5.722. [DOI] [PubMed] [Google Scholar]
  6. Brown M. S., Goldstein J. L. How LDL receptors influence cholesterol and atherosclerosis. Sci Am. 1984 Nov;251(5):58–66. doi: 10.1038/scientificamerican1184-58. [DOI] [PubMed] [Google Scholar]
  7. Brown M. S., Goldstein J. L. Lipoprotein receptors in the liver. Control signals for plasma cholesterol traffic. J Clin Invest. 1983 Sep;72(3):743–747. doi: 10.1172/JCI111044. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chevallier F. Dynamics of cholesterol in rats, studied by the isotopic equilibrium method. Adv Lipid Res. 1967;5:209–239. doi: 10.1016/b978-1-4831-9941-2.50012-1. [DOI] [PubMed] [Google Scholar]
  9. DOLE V. P. A relation between non-esterified fatty acids in plasma and the metabolism of glucose. J Clin Invest. 1956 Feb;35(2):150–154. doi: 10.1172/JCI103259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Davis R. A., Hartman A. D., Dory L., Van Lenten B. J., Roheim P. S. Metabolic fate of VLDL apolipoproteins B and E in hepatectomized rats. Biochim Biophys Acta. 1981 Jul 24;665(1):154–164. doi: 10.1016/0005-2760(81)90243-5. [DOI] [PubMed] [Google Scholar]
  11. Dietschy J. M., Spady D. K., Stange E. F. Quantitative importance of different organs for cholesterol synthesis and low-density-lipoprotein degradation. Biochem Soc Trans. 1983 Dec;11(6):639–641. doi: 10.1042/bst0110639. [DOI] [PubMed] [Google Scholar]
  12. GOODMAN D. S. The metabolism of chylomicron cholesterol ester in the rat. J Clin Invest. 1962 Oct;41:1886–1896. doi: 10.1172/JCI104645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. GOULD R. G., LEROY G. V., OKITA G. T., KABARA J. J., KEEGAN P., BERGENSTAL D. M. The use of C14-labeled acetate to study cholesterol metabolism in man. J Lab Clin Med. 1955 Sep;46(3):372–384. [PubMed] [Google Scholar]
  14. Hamilton R. L., Wong J. S., Guo L. S., Krisans S., Havel R. J. Apolipoprotein E localization in rat hepatocytes by immunogold labeling of cryothin sections. J Lipid Res. 1990 Sep;31(9):1589–1603. [PubMed] [Google Scholar]
  15. Havel R. J. Lowering cholesterol, 1988. Rationale, mechanisms, and means. J Clin Invest. 1988 Jun;81(6):1653–1660. doi: 10.1172/JCI113501. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  17. MATTHEWS C. M. Effects of plasmapheresis on albumin pools in rabbits. J Clin Invest. 1961 Mar;40:603–610. doi: 10.1172/JCI104290. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Quarfordt S. H., DeFaria E., Landis B. A., Bollinger R. R., Yamaguchi Y. Transport of free fatty acid and triglyceride in anhepatic rats. Hepatology. 1991 Nov;14(5):911–919. doi: 10.1002/hep.1840140526. [DOI] [PubMed] [Google Scholar]
  19. Quarfordt S. H., Hilderman H. L. Quantitation of the in vitro free cholesterol exchange of human red cells and lipoproteins. J Lipid Res. 1970 Nov;11(6):528–535. [PubMed] [Google Scholar]
  20. Redgrave T. G. Formation of cholesteryl ester-rich particulate lipid during metabolism of chylomicrons. J Clin Invest. 1970 Mar;49(3):465–471. doi: 10.1172/JCI106255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Renaud G., Hamilton R. L., Havel R. J. Hepatic metabolism of colloidal gold-low-density lipoprotein complexes in the rat: evidence for bulk excretion of lysosomal contents into bile. Hepatology. 1989 Mar;9(3):380–392. doi: 10.1002/hep.1840090307. [DOI] [PubMed] [Google Scholar]
  22. Shelburne F. A., Quarfordt S. H. The interaction of heparin with an apoprotein of human very low density lipoprotein. J Clin Invest. 1977 Oct;60(4):944–950. doi: 10.1172/JCI108849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shelburne F., Hanks J., Meyers W., Quarfordt S. Effect of apoproteins on hepatic uptake of triglyceride emulsions in the rat. J Clin Invest. 1980 Mar;65(3):652–658. doi: 10.1172/JCI109710. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  25. Yamaguchi Y., Bollinger R. R., DeFaria E., Landis B., Quarfordt S. A simplified single stage total hepatectomy in the rat with maintenance of gastrointestinal absorptive function. Hepatology. 1989 Jan;9(1):69–74. doi: 10.1002/hep.1840090111. [DOI] [PubMed] [Google Scholar]

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