Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 8;34(34):4531–4544. doi: 10.1038/onc.2014.388

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

ERM proteins localise at invadopodia adhesion rings and mediate podoplanin ring assembly. (a, b) Specific localisation of moesin (a) and ezrin (b) at invadopodia of HN5 cells cultured on crosslinked gelatin. Note that ezrin and moesin cluster to invadopodia adhesion rings where they colocalise with podoplanin. Graphs indicate fluorescent intensity (in arbitrary units) of each marker over the indicated line scan. Data shown are representative from five invadopodia analysed per condition from three independent experiments. Bars=10 μm (upper panels) and 5 μm (lower panels). (c) Western blot analysis of ezrin, moesin and podoplanin expression in HN5 cells upon specific siRNA treatment. (d) Gelatin-degradation assay (6 h) of HN5 cells treated with ERM and podoplanin siRNAs. Bars=20 μm. (e) Quantification of the gelatin-degradation assay depicted in d. The results shown are the means±s.e.m. of n⩾100 cells for each condition over three independent experiments. ***P<0.0005; **P<0.005; *P<0.05.