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Platelets were preincubated with nifedipine or nifedipine combined with GSK0660 or GW9662 for 3 min; then NBT or DCFH-DA was added, followed by collagen to measure superoxide (A) and hydrogen peroxide (B) formation respectively. Data were expressed as means ± SEM (n = 5). *** p < 0.001 compared with collagen alone group; ++ p < 0.01 compared with respective collagen+nifedipine treated group.
