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LARP1 knockdown decreases mTOR protein level and its downstream targets P-RPS6 and P-4EBP in HeLa and PC9 cell lines. Western blotting analysis of LARP1 in HeLa (a) and PC9 (b) cell lines after LARP1 knockdown using two independent siRNA oligos showing decrease in expression of mTOR, P-4EBP and P-RPS6, but not total RPS6 ribosomal protein. Total 4EBP showed a moderate decrease in HeLa cells. HSP60 was used as a loading control.
