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. Author manuscript; available in PMC: 2016 Mar 16.
Published in final edited form as: Mol Biochem Parasitol. 2015 Mar 16;199(0):9–18. doi: 10.1016/j.molbiopara.2015.03.002

Fig. 4.

Fig. 4

Effects of TbTim50 up- and down-regulations on cell injury during H2O2 treatment. TbTim50 KD and TbTim50 OE cells were grown in the presence of doxycycline for 4 days. Parental control T. brucei were also grown in parallel. (A) Cells were treated with different concentration of H2O2 (0–800 μM) for 2 h, washed with PBS and stained with sytox green and resorufin as described. FACS analysis was performed immediately and data were analyzed by Flowjo software. FACS analysis of the unstained, heat killed (Dead) and live parasites were performed in parallel. (B) Ratios of the live and injured cell (%) were calculated from three independent experiments and plotted versus the different cell type at different concentrations of H2O2. Values shown are mean ± standard errors from triplicate samples.